A SIMPLE SECTIONING TECHNIQUE FOR ELECTRON MICROSCOPY

Uniform serial sectioning for transmission electron microscopy.

Kristen M. Harris,1,2,3 Elizabeth Perry,2 Jennifer Bourne,1,2 Marcia Feinberg,3 Linnaea Ostroff,3 and Jamie Hurlburt2,3 1Section of Neurobiology and Center for Learning and Memory, University of Texas at Austin, Austin, Texas 78712, 2Department of Neurology, Synapses, and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia 30912, 3Program in Neuroscience and Biology Depa...

Sectioning of tissue for electron microscopy.

A model based reconstruction technique for depth sectioning with scanning transmission electron microscopy

Depth sectioning in high angular annular dark field scanning transmission electron microscopy is considered a candidate for three-dimensional characterization on the atomic scale. However at present the depth resolution is still far from the atomic level, due to strong limitations in the opening angle of the beam. In this paper we introduce a new, parameter based tomographic reconstruction algo...

A Simple Freeze Fracture Technique for Scanning Electron Microscopy of Collagenous Biomaterials

A simple freeze fracture technique for the ultrastructural analysis of fragile collagenous biomaterials is presented. Following standard methods of preparation and dehydration, fragile biomaterials are fractured with a sharp blade attached to a modified slide hammer. The sliding mass imparts a sudden impact load on the frozen surface of the material , creating a distortion free cleavage plane a...

Nucleopolyhedrovirus: scanning electron microscopy technique.

A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infect...